Improvement of enzyme activity, stability and selectivity via immobilization techniques C Mateo, JM Palomo, G Fernandez-Lorente, JM Guisan, ... Enzyme and microbial technology 40 (6), 1451-1463, 2007 | 3940 | 2007 |
Immobilization of lipases by selective adsorption on hydrophobic supports R Fernandez-Lafuente, P Armisén, P Sabuquillo, G Fernández-Lorente, ... Chemistry and physics of lipids 93 (1-2), 185-197, 1998 | 622 | 1998 |
Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases JM Palomo, G Muñoz, G Fernández-Lorente, C Mateo, ... Journal of Molecular Catalysis B: Enzymatic 19, 279-286, 2002 | 491 | 2002 |
Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins C Mateo, JM Palomo, M Fuentes, L Betancor, V Grazu, F López-Gallego, ... Enzyme and Microbial Technology 39 (2), 274-280, 2006 | 462 | 2006 |
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ... Biotechnology progress 18 (3), 629-634, 2002 | 419 | 2002 |
Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their … C Mateo, G Fernández-Lorente, O Abian, R Fernández-Lafuente, ... Biomacromolecules 1 (4), 739-745, 2000 | 405 | 2000 |
Some special features of glyoxyl supports to immobilize proteins C Mateo, O Abian, M Bernedo, E Cuenca, M Fuentes, ... Enzyme and Microbial Technology 37 (4), 456-462, 2005 | 350 | 2005 |
Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method C Mateo, R Torres, G Fernández-Lorente, C Ortiz, M Fuentes, A Hidalgo, ... Biomacromolecules 4 (3), 772-777, 2003 | 305 | 2003 |
General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality JM Palomo, M Fuentes, G Fernández-Lorente, C Mateo, JM Guisan, ... Biomacromolecules 4 (1), 1-6, 2003 | 279 | 2003 |
Interfacially activated lipases against hydrophobic supports: Effect of the support nature on the biocatalytic properties G Fernandez-Lorente, Z Cabrera, C Godoy, R Fernandez-Lafuente, ... Process Biochemistry 43 (10), 1061-1067, 2008 | 246 | 2008 |
Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements C Carrasco-López, C Godoy, B de Las Rivas, G Fernández-Lorente, ... Journal of Biological Chemistry 284 (7), 4365-4372, 2009 | 241 | 2009 |
Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters JM Palomo, G Fernandez-Lorente, C Mateo, C Ortiz, ... Enzyme and Microbial Technology 31 (6), 775-783, 2002 | 222 | 2002 |
Novozym 435 displays very different selectivity compared to lipase from Candida antarctica B adsorbed on other hydrophobic supports Z Cabrera, G Fernandez-Lorente, R Fernandez-Lafuente, JM Palomo, ... Journal of Molecular Catalysis B: Enzymatic 57 (1-4), 171-176, 2009 | 212 | 2009 |
Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering. Kinetic resolution of (±)-α-hydroxy-phenylacetic acid derivatives JM Palomo, G Fernández-Lorente, C Mateo, M Fuentes, ... Tetrahedron: Asymmetry 13 (12), 1337-1345, 2002 | 170 | 2002 |
CLEAs of lipases and poly-ionic polymers: A simple way of preparing stable biocatalysts with improved properties L Wilson, G Fernández-Lorente, R Fernández-Lafuente, A Illanes, ... Enzyme and Microbial Technology 39 (4), 750-755, 2006 | 150 | 2006 |
Use of immobilized lipases for lipase purification via specific lipase–lipase interactions JM Palomo, C Ortiz, M Fuentes, G Fernandez-Lorente, JM Guisan, ... Journal of Chromatography A 1038 (1-2), 267-273, 2004 | 148 | 2004 |
Self‐assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties G Fernández‐Lorente, JM Palomo, M Fuentes, C Mateo, JM Guisán, ... Biotechnology and bioengineering 82 (2), 232-237, 2003 | 148 | 2003 |
Lipase–lipase interactions as a new tool to immobilize and modulate the lipase properties JM Palomo, C Ortiz, G Fernández-Lorente, M Fuentes, JM Guisán, ... Enzyme and microbial technology 36 (4), 447-454, 2005 | 147 | 2005 |
One‐step purification, covalent immobilization, and additional stabilization of poly‐His‐tagged proteins using novel heterofunctional chelate‐epoxy supports C Mateo, G Fernández‐Lorente, E Cortés, JL Garcia, ... Biotechnology and Bioengineering 76 (3), 269-276, 2001 | 144 | 2001 |
Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance G Fernández-Lorente, JM Palomo, C Mateo, R Munilla, C Ortiz, Z Cabrera, ... Biomacromolecules 7 (9), 2610-2615, 2006 | 142 | 2006 |