Jan-Willem de Gier
Jan-Willem de Gier
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Tuning Escherichia coli for membrane protein overexpression
S Wagner, MM Klepsch, S Schlegel, A Appel, R Draheim, M Tarry, ...
Proceedings of the National Academy of Sciences 105 (38), 14371-14376, 2008
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
PA Scotti, ML Urbanus, J Brunner, JWL de Gier, G von Heijne, ...
The EMBO journal, 2000
Consequences of membrane protein overexpression in Escherichia coli
S Wagner, L Baars, AJ Ytterberg, A Klussmeier, CS Wagner, O Nord, ...
Molecular & Cellular Proteomics 6 (9), 1527-1550, 2007
Optimization of membrane protein overexpression and purification using GFP fusions
D Drew, M Lerch, E Kunji, DJ Slotboom, JW de Gier
Nature methods 3 (4), 303-313, 2006
Rationalizing membrane protein overexpression
S Wagner, ML Bader, D Drew, JW de Gier
Trends in biotechnology 24 (8), 364-371, 2006
The Escherichia coli SRP and SecB targeting pathways converge at the translocon
QA Valent, PA Scotti, S High, JWL de Gier, G Von Heijne, G Lentzen, ...
The EMBO journal, 1998
Competition between Sec‐and TAT‐dependent protein translocation in Escherichia coli
S Cristóbal, JW de Gier, H Nielsen, G von Heijne
The EMBO journal, 1999
J Luirink, G Heijne, E Houben, JW Gier
Annu. Rev. Microbiol. 59, 329-355, 2005
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli
DE Drew, G Von Heijne, P Nordlund, JWL de Gier
FEBS letters 507 (2), 220-224, 2001
The heme-copper oxidase family consists of three distinct types of terminal oxidases and is related to nitric oxide reductase
J van der Oost, APN De Boer, JWL de Gier, WG Zumft, AH Stouthamer, ...
FEMS microbiology letters 121 (1), 1-9, 1994
Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
D Drew, D Sjöstrand, J Nilsson, T Urbig, C Chin, JW de Gier, ...
Proceedings of the National Academy of Sciences 99 (5), 2690-2695, 2002
Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC
ML Urbanus, PA Scotti, L Fröderberg, A Sääf, JWL de Gier, J Brunner, ...
EMBO reports 2 (6), 524-529, 2001
Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
QA Valent, JWL De Gier, G Heijne, DA Kendall, CM Ten Hagen‐Jongman, ...
Molecular microbiology 25 (1), 53-64, 1997
Assembly and overexpression of membrane proteins in Escherichia coli
D Drew, L Fröderberg, L Baars, JWL de Gier
Biochimica et Biophysica Acta (BBA)-Biomembranes 1610 (1), 3-10, 2003
Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
JWL de Gier, P Mansournia, QA Valent, GJ Phillips, J Luirink, ...
FEBS letters 399 (3), 307-309, 1996
Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21 (DE3)
S Schlegel, J Löfblom, C Lee, A Hjelm, M Klepsch, M Strous, D Drew, ...
Journal of molecular biology 423 (4), 648-659, 2012
A scalable, GFP‐based pipeline for membrane protein overexpression screening and purification
D Drew, DJ Slotboom, G Friso, T Reda, P Genevaux, M Rapp, ...
Protein science 14 (8), 2011-2017, 2005
YidC/Oxa1p/Alb3: evolutionarily conserved mediators of membrane protein assembly
J Luirink, T Samuelsson, JW de Gier
FEBS letters 501 (1), 1-5, 2001
Function of YidC for the insertion of M13 procoat protein inEscherichia coli: Translocation of mutants that show differences in their membrane potential dependence and sec …
JC Samuelson, F Jiang, L Yi, M Chen, JW de Gier, A Kuhn, RE Dalbey
Journal of Biological Chemistry 276 (37), 34847-34852, 2001
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
JWL De Gier, PA Scotti, A Sääf, QA Valent, A Kuhn, J Luirink, ...
Proceedings of the National Academy of Sciences 95 (25), 14646-14651, 1998
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